As will be shown below, the configurability of the correlator is key to measuring otherwise difficult heterogeneous The challenge in this case is choosing the appropriate correlator layout Partially aggregated protein might span 1-2 orders of magnitude in hydrodynamic Sizes on the order of several hundred nanometers or greater. Protein aggregates can easily reach large A high-speed correlator is necessary to measure the size Protein monomers are typically < 10 nm, with many as Many common globular proteins are very small in This is the basis for measuring a particle size distribution. The signal that arises from the scattered intensity from the laser light is acquired and transformed into an autocorrelation function. These fluctuations are directly related to the motion of particles. In order to demonstrate this principle, we compare aggregation studies conducted on pharmaceutical-grade monoclonal antibody (mAb), with results obtained for a common protein, serum albumin (BSA).Ĭheck out our informative video on Monoclonal Antibodies and DLS IntroductionĭLS relies on the principle that freely diffusing material, moving randomly due to Brownian motion, will produce rapid fluctuations in scattered laser light. The timescale of these intensity fluctuations is on the order of tens of nanoseconds to hundreds of milliseconds.
#Dynamic light scattering continuous full#
When DLS is used to its full potential, aggregation of proteins can be detected and the size of monomeric protein can be determined to a very high degree of certainty. Failing to adhere to these strict and exacting standards compromises the processability, activity, and shelf stability of antibody-based products. In all these applications it is essential that antibodies remain intact, and monomeric. These highly versatile proteins are used as the major functional element in immunoassays and other rapid diagnostics, in vaccine production, and as the primary component of a wide array of injectable protein drugs. One of the fastest growing classes of pharmaceutically active biologics are antibodies. Please follow the link to our light scattering theory page to learn more about dynamic and static light scattering theory.Dynamic light scattering (DLS) is a powerful technique that is amenable to measurement of biological samples, particularly proteins and biopolymers. Owing to the technique’s high sensitivity towards large species, even small impurities caused by aggregation can be detected Owing to its large measurement range (0.5 nm – 1 µm) DLS closes the gap between the range of small soluble aggregates that are detected via size exclusion chromatography (SEC) and particles analyzed by methods such as light obscuration (LO) or micro flow imaging (MFI). ZentriForce Pharma offers DLS services under BSL 1 and 2.ĭynamic light scattering is known as a fast and sensitive screening method for aggregate / particle detection and therefore widely used within biopharmaceutical development. We routinely conduct biosimilarity and gene therapy studies and are furthermore experienced in the development of dynamic light scattering (DLS) methods for a large variety of samples. Zentriforce Pharma is a contract research organization that specializes in analytical services with a focus on hydrodynamic methods. Asymmetrical Flow Field-Flow Fractionation (AF4) Theory.Sedimentation Equilibrium Analytical Ultracentrifugation (SE-AUC).Sedimentation Velocity Analytical Ultracentrifugation (SV-AUC) Data Evaluation Approaches.Sedimentation Velocity Analytical Ultracentrifugation (SV-AUC).DLS Services for the Development of other Gene Therapy APIs.AF4 Services for the Development of other Gene Therapy APIs.SV-AUC Services for the Development of other Gene Therapy APIs.SEC-MALLS Services for Peptide Development.SV-AUC Services for Peptide Development.SEC-MALLS Services for Biopharmaceutical Development.DLS Services for Biopharmaceutical Development.AF4 Services for Biopharmaceutical Development.SV-AUC Services for Biopharmaceutical Development.SEC-MALLS Services for Biosimilarity Studies.
SV-AUC Services for Biosimilarity Studies.Dynamic Light Scattering (DLS) Services.Differential Scanning Calorimetry (μ-DSC) Services.Size Exclusion Chromatography (SEC-MALLS) Services.Asymmetrical Flow Field-Flow Fractionation (AF4) Services.Sedimentation Velocity Analytical Ultracentrifugation (SV-AUC) Services.
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